Unexpected Activities in Regulating Ciliation Contribute to Off-target Effects of Targeted Drugs

Controversies in Targeted Therapy of Adult T Cell Leukemia/Lymphoma: ON Target or OFF Target Effects?

Adult T cell leukemia/lymphoma (ATL) represents an ideal model for targeted therapy because of intrinsic chemo-resistance of ATL cells and the presence of two well identified targets: the HTLV-I retrovirus and the viral oncoprotein Tax. The combination of zidovudine (AZT) and interferon-alpha (IFN) has a dramatic impact on survival of ATL patients. Although the mechanism of action remains uncle...

Questioning unexpected CRISPR off-target mutations in vivo

To the Editor: Recently, Schaefer et al.1 reported that whole genome sequencing (WGS) of two Cas9-treated, gene-corrected mice and a wild-type control mouse unveiled 1,397 singlenucleotide variations (SNVs) and 117 small insertions and deletions (indels) present commonly in the two Cas9-treated mice “but absent in the uncorrected control” and from a database of mouse SNVs and indels. There was ...

Use of Patch Clamp Electrophysiology to Identify Off-Target Effects of Clinically Used Drugs

In Silico Investigation of Off-Target Effects

Off-Target Effects of Psychoactive Drugs Revealed by Genome-Wide Assays in Yeast

To better understand off-target effects of widely prescribed psychoactive drugs, we performed a comprehensive series of chemogenomic screens using the budding yeast Saccharomyces cerevisiae as a model system. Because the known human targets of these drugs do not exist in yeast, we could employ the yeast gene deletion collections and parallel fitness profiling to explore potential off-target eff...